Journal: The New Phytologist

Article Title: Antisense reduction in NADP ‐ ME in the C 4 species Flaveria bidentis alters stomatal sensitivity to intercellular [ CO 2 ]

doi: 10.1111/nph.71012

Figure Lengend Snippet: Stomatal conductance and intercellular CO 2 concentration at two spectral light conditions in wild‐type (WT) plants of C 4 Flaveria bidentis and plants expressing an antisense construct against NADP‐malic enzyme. CO 2 assimilation rate (A; a, b, c), Stomatal conductance ( g s ; d, e, f), and intercellular CO 2 concentration (C i ; g, h, i) in WT‐LL and three antisense lines of C 4 F. bidentis at 5, 65, and 125 mins under specified light conditions. Boxes show interquartile range, horizontal lines show median and whiskers show data range. Full timeseries are provided in Supporting Information Fig. . Significant differences in A, g s , and C i were determined using model‐estimated means with Tukey's Honestly Significant Difference adjustment at α = 0.05. Within each C i (g–i) time point panel, box plots sharing the same letter are not significantly different. Diamonds indicate group means ( n = 4).

Article Snippet: All subsequent steps were performed on the blotted membranes under gentle agitation (60–100 rpm) using a horizontal shaker (Ultimax 1010; Heidolph Instruments, Schwabach, Germany).

Techniques: Concentration Assay, Expressing, Construct

Journal: The New Phytologist

Article Title: Antisense reduction in NADP ‐ ME in the C 4 species Flaveria bidentis alters stomatal sensitivity to intercellular [ CO 2 ]

doi: 10.1111/nph.71012

Figure Lengend Snippet: Stomatal conductance in response to varying light intensity, while keeping C i constant in wild‐type (WT) plants of C 4 Flaveria bidentis and plants expressing an antisense construct against NADP‐malic enzyme (NADP‐ME). Response of stomatal conductance ( g s ; a), CO 2 assimilation rate ( A n ; b), and intercellular CO 2 concentration (Cᵢ; c) in WT and independent NADP‐ME antisense lines of C 4 F. bidentis were measured across increasing red light photon flux densities at a common Cᵢ of 120 μmol CO 2 mol −1 , matching the WT C i . Boxes show interquartile range, horizontal lines show median and whiskers show data range. Each box plot represents 4 to 6 biological replicates, with means indicated by black diamonds. Statistical groupings (letters) reflect differences among genotypes as determined by a linear mixed‐effects model accounting for replicate effects and evaluated across all light levels.

Article Snippet: All subsequent steps were performed on the blotted membranes under gentle agitation (60–100 rpm) using a horizontal shaker (Ultimax 1010; Heidolph Instruments, Schwabach, Germany).

Techniques: Expressing, Construct, Concentration Assay